smai restriction enzyme (New England Biolabs)
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smai restriction enzyme
Smai Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smai+restriction+enzyme/SmaI/pmc13049938-281-0-5
Average 97 stars, based on 2722 article reviews
Smai Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smai+restriction+enzyme/SmaI/pmc13049938-281-0-5
Average 97 stars, based on 2722 article reviews
smai restriction enzyme - by Bioz Stars,
2026-09
97/100 stars
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1) Product Images from "Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression"
Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
Journal: Molecular Therapy. Nucleic Acids
doi: 10.1016/j.omtn.2026.102899
Figure Legend Snippet: Recombinant AAV genome element and heterogeneity analysis by native and alkaline electrophoresis (A) Graphic representation of the main recombinant AAV vector genome elements evaluated: wtITR (143 nt, black) versus dITR/wtITR (11-nt deletion in the 5′ C arm, 130 nt, red), EAlbAAT (albumin enhancer-alpha1-antitrypsin promoter, blue), Egfp (enhanced green fluorescent protein, green), pA (bovine/human growth hormone polyadenylation signal, yellow), and CBA (CMV (cytomegalovirus) enhancer-chicken beta actin promoter, orange). Vector genome sizes are shown next to each representation. (B) Native automated electrophoresis of extracted rAAV genomes from dITR/wtITR and wtITR. EalbAAT . Egfp vectors by TapeStation. From left to right: AAV2, AAV3B, AAV5, AAV8, AAV9, and AAVLK03 serotypes. As a run reference, the upper marker (10,000 bp) and lower marker (15 bp) of the ladder are highlighted in purple and green, respectively. (C) Native automated electrophoresis of extracted rAAV genomes from AAV5 and AAV8 serotypes with dITR vs. wtITR and CBA versus EalbAAT promoters. As a run reference, the upper marker (10,000 bp) and lower marker (15 bp) of the ladder are highlighted in purple and green, respectively. (D) Native automated electrophoresis of extracted rAAV genomes from the AAVLK03 serotype with dITR and CBA vs. EalbAAT promoters. As a run reference, the upper marker (10,000 bp) and lower marker (15 bp) of the ladder are highlighted in purple and green, respectively. (E) Native electrophoresis of extracted rAAV genomes from AAV9 and AAVLK03 serotypes with dITR and CBA versus EalbAAT promoters, before and after digestion at 37°C for 1 h with SmaI. (F) Graphic representation of the dITR/tITR design (11-nt deletion in the 5′ C arm and 22-nt deletion of the 3′ B arm, 121 nt, purple), scITR (C arm deletion, 106 nt, green), and the dITR. AAT . Egfp expression cassette including an hypoxanthine phosphoribosyltransferase 1 ( HPRT 1 ) DNA stuffer sequence (GenBank: NG_012329.2 ) (bottom). Vector genome sizes are shown next to each design. (G) Alkaline electrophoresis of AAV8 vectors with different ITR configurations and CBA versus EalbAAT promoters. (H) Alkaline electrophoresis of AAV8 and AAV3B vectors with dITR/wtITR, dITR/tITR, and scITR configurations. (I) Alkaline electrophoresis of AAV8 and AAVLK03 vectors with the dITR/tITR configuration. (J) Alkaline electrophoresis of AAV3B, AAV5, and AAVLK03 vectors with dITR/wtITR configuration and CBA versus EalbAAT promoters. Combinations are displayed on top of each lane. Agarose gel ladder: FastGene 1 kb DNA marker (10,000–100 bp).
Techniques Used: Recombinant, Electrophoresis, Plasmid Preparation, Marker, Expressing, Sequencing, Agarose Gel Electrophoresis
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